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From: Altered energy metabolism and metabolic gene expression associated with increased metastatic capacity identified in MDA-MB-231 cell line variants

Altered energy metabolism and metabolic gene expression associated with increased metastatic capacity identified in MDA-MB-231 cell line variants

Figure 1. Proliferation of the highly metastatic MDA-MB-231HM.LNm5 (MDA-231HM.LNm5) and parental MDA-MB-231_ATCC (MDA-231) cell lines. Cell growth in the presence and absence of fetal calf serum (FCS) (5% or 10%) after 24 h serum starvation was measured by enumeration of viable cells (tryplan blue exclusion) (A) or the resazurin fluorometric method (B). Mitochondrial metabolic activity (C) was also determined [total relative fluorescent units (RFU) divided by the total number of cells]. Data is presented as mean ± SEM, n = 4. Two-way ANOVA with Bonferroni’s post hoc test was used to test for statistical significance *P < 0.05; **P < 0.01; ***P < 0.001 vs. MDA-231 control; ^P < 0.05; ^^^P < 0.001 vs. MDA-231HM.LNm5 control. BSA: bovine serum albumin; NS: not significant