fig3

Figure 3. Flowchart for MSI analysis of interstitial fluid, urine and blood plasma samples on AuNPET target. Step 1: semi-manual application of diluted samples onto AuNPET target with the aid of 3D translation stage; 2: programming of four-pixel array per sample spot; 3, 4, 5: LDI MS measurements in imaging mode producing series of spectra; 6: data processing. MSI: mass spectrometry imaging; AuNPET: gold nanoparticle-enhanced target; SALDI: surface-assisted, laser desorption ionization; LDI: laser desorption/ionization