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![Two sides to colon cancer: mice mimic human anatomical region disparity in colon cancer development and progression](https://image.oaes.cc/454cb2fe-c1eb-4f09-aebe-4ae891319a79/2807.fig.1.png)
Figure 1. Main steps in the surgical approach to injecting colon cancer cells in the murine distal colon. A: Isolation of the distal colon (outlined) using moist sterile cotton tip applicators with retraction of the abdominal wall and evisceration of abdominal organs; B: injection of 5 × 106 HT-29 human colon cancer cells into the wall of the distal colon (arrow) using a 27-guage needle; C: applying pressure with a moist sterile cotton tip applicator at the injection site to prevent leakage and hemorrhage