fig5
From: Increased ARF6 activation correlates with HGF stimulation in non-invasive prostate cancer cells
Figure 5. A: GST pull down assay for ARF6-GTP was performed on prostate cells from normal prostate (PNT2), localised prostate cancer (CAHVP-10), weakly metastatic (LNCaP), moderately (DU145) and aggressively (PC-3) metastatic prostate cancer. Twenty five microlitres of GST assay elute was used in western blot analysis for ARF6-GTP detection. A: corresponding 25 µL aliquot from total protein lysis was used for detection of total ARF6 protein; B: band density was measured by densitometry and the ratios of ARF6-GTP to total ARF6 are shown. The data are expressed as mean (from triplicate experiments) ± SD. *Denotes significant difference compared to PNT2 (P < 0.05)
