fig3
Figure 3. Gene expression analysis shows ARF6 is expressed in prostate cells derived from normal tissue (PNT2), localised prostate cancer (CAHVP-10), weakly metastatic (LNCaP), moderately (DU145) and aggressively (PC-3) metastatic prostate cancer tissue. ARF6 expression was similar between PNT2 and CAHPV-10. A significant increase in expression was observed in LNCaP, DU145 and PC-3 compared to CAHPV-10. The data are expressed as mean (from triplicate experiments), relative to PNT2, ± SD. *Denotes significant difference compared to PNT2 (P < 0.05); B: western blot analysis for total ARF6 protein; C: densitometry data is presented relative to the level of β-actin. The data are expressed as mean (from triplicate experiments) ± SD