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Figure 2. Baseline mRNA expression of EGFR-mutation positive cells and sensitivity to PIM inhibition. A: baseline mRNA expression of various genes in five EGFR-mutation-positive NSCLC cell lines (H1975, PC9, 11-18, HCC4006 and HCC827). Heatmap depicts gene mRNA expression (columns) in the different cell lines (rows) compared to the average mRNA expression of each gene in all cell lines. Experiments were performed in biological triplicates, and the average of these experiments are shown; B: cell viability assays were performed in all cell lines, to explore the effect of AZD1208 in vitro, and to determine the IC50 in each cell line. Results of previously performed experiments with osimertinib in the same cell lines are also shown. Experiments were performed in biological triplicates, and the average and standard deviations are shown. EGFR: epidermal growth factor receptor; PIM: proviral integration site for Moloney murine leukemia virus; NSCLC: non-small cell lung cancer; IC₅₀: half maximal inhibitory concentration; JAK2: Janus kinase 2; STAT3: signal transducer and activator of transcription 3; SHP2 (PTPN11): tyrosine-protein phosphatase non-receptor type 11