fig6
![Differential expression and function of the endogenous lactate receptor, GPR81, in ERα-positive/HER2-positive epithelial <i>vs</i>. post-EMT triple-negative mesenchymal breast cancer cells](https://image.oaes.cc/bbc74684-e6ee-4408-9666-4d209d341f91/3101.fig.6.jpg)
Figure 6. Additive effect of GPR81 knockdown and Tamoxifen treatment in reducing the cell proliferation and increasing cell apoptosis in epithelial MCF-7 breast cancer cells. MCF-7-siNT (control) and MCF-7-siGPR81 cancer cells were treated with or without 1 μmol/L Tamoxifen for 96 h in 3-dimensional Matrigel with physiological modified medium. A: real-time qPCR analysis of lactate importer MCT1; and progesterone receptor PR; B: relative mRNA expression of proliferation markers: Ki67 and Cyclin B1; C: viable cell counts of epithelial MCF-7-siNT and MCF-7-siGPR81; D: Western blot analysis of cell lysates from MCF-7-siNT and MCF-7-siGPR81 used to detect protein expression levels of the apoptotic maker cleaved-PARP. β-actin was used as a loading control. Results are presented as mean ± S.E.M. of three independent experiments *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (ordinary one-way ANOVA)