fig3

Figure 3. GPR81 regulates expression of the gene involved in lactate import in MCF-7 epithelial breast cancer cell line. A: real-time qPCR analysis of GPR81; B: MCT1 and MCT4 mRNA levels in MCF-7-siNT (control) and MCF-7-siGPR81 cultured in 3-dimensional Matrigel with physiological modified medium at 72 and 96 h after transfection; C: Western blot analysis of cell lysates from MCF-7-siNT and MCF-7-siGPR81 cells used to detect protein expression levels of MCT1 and MCT4. β-actin was used as a loading control. The bars represent the mean ± S.E.M of three independent experiments; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (ordinary one-way ANOVA)