fig1
Figure 1. GPR81 expression of epithelial MCF-7 and BT-474 cells and mesenchymal MCF-7M and BT-474M in 3-dimensional Matrigel culture. A: fluorescence microscopy images of 3-dimensional structures of proliferating epithelial (E) and mesenchymal (M) cells in 3-dimensional Matrigel with physiological modified medium for 7 days. Green, Phalloidin staining of F-actin; red/orange, nuclei counterstained with SYTOXTM orange nucleic acid (red); B: real-time qPCR analysis of GPR81 in epithelial (E) and mesenchymal (M) cells. Results are presented as mean ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (ordinary one-way ANOVA)
