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Supplementary Figure 4. TGF-β regulates EGFR and IGF-1R expression in BT474 cells. (A) BT474 cells were stimulated with TGF-β1 (5 ng/mL) for 0-96 h as indicated, at which point differences in EGFR expression were monitored by real-time PCR. (B) Differential mRNA expression of EGFR and IGF-1R in quiescent BT474 cells. Data are the mean fold-changes (± SE; n = 3; *P < 0.05; Student's t-test). TGF: transforming growth factor; IGF: insulin-like growth factor; EGFR: epidermal growth factor receptor; SE: standard error