fig3
![Cancer immunity and therapy using hyperthermia with immunotherapy, radiotherapy, chemotherapy, and surgery](https://image.oaes.cc/3a9b9e2a-7270-4e98-92a4-841069ff3d9c/2272.fig.3.png)
Figure 3. Whole-body hyperthermia stimulates IFN-γ and IL-2 production from T-cells. Blood was obtained from donors before (Pre), immediately after (Post), 24 h after (24 h), and 48 h after (48 h) whole-body hyperthermia. PBMCs from each time point were extracted and co-cultured with monoclonal antibodies against CD3 and CD28 to measure IFN-γ and IL-2 production levels in each supernatant. Results are shown as fold over the control (Pre) for the average of five separate donors, and expressed as mean ± SEM. Statistical differences from control was evaluated using paired t-test. P < 0.05 was recognized statistically significant[42]. IFN: interferon; IL: interleukin; PBMCs: peripheral blood mononuclear cells