fig3

Figure 3. Expression of IL-1β, IFN-γ, HMOX-1 and GNLY genes was significantly different between BCG responders and relapsing patients at week 6. Relative mRNA levels of IL-1β, IFN-γ, HMOX-1 and GNLY were evaluated by real time PCR, as described in the Methods section. mRNA was obtained from blood samples of BCG responder and relapsing (rel-) patients, collected before (6WBT) and 24 h after (6WAT) BCG treatment performed 6 weeks after TURBT. Values were calculated by formula 2^-∆Ct *1,000 and infer the number of mRNA molecules of a certain gene per 1,000 molecules of the average of the endogenous control (β-actin). Statistical significances (*P < 0.05) refer to differences between samples from BCG responders and relapsing patients collected before (6WBT) or 24 h after (6WAT) BCG 6 weeks treatment. BCG: Bacille Calmette-Guérin; PCR: polymerase chain reaction; TURBT: transurethral resection of the bladder tumor