fig2

Figure 2. Because the chondrocytes were from porcine and the ADSCs were from human, we determined the contribution of each cell type to chondrogenesis. Hy-PYD assesses collagen crosslinking. Here, the expression of Hy-PYD was assessed from the discs by ELISA, and the 1:9 condition increased the expression over the chondrocytes alone. The 1:9 condition was significantly greater than the 1:5 condition. This indicated that ADSCs were stimulating the chondrocytes to be more chondrogenic. ADSCs: adipose derived stem cells; ELISA: enzyme-linked immunosorbent assay