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![Proliferation and adipogenic differentiation of human adipose-derived stem cells isolated from middle-aged patients with prominent orbital fat in the lower eyelids](https://image.oaes.cc/53ccf777-d567-4054-871f-7e4069cc8015/1786.fig.3.png)
Figure 3. Adipogenic differentiation was assessed by oil red O staining after culturing orbital adipose-derived stem cells in adipogenic media for 14 days. (A, C): young group; (B, D): middle-aged group; (A, B): non-induced; (C, D): induced. More positive staining cells were observed in (A, C) than those in (B, D), and almost no staining was seen in (A, B) (scale bar: 100 µm)