fig8

Figure 8. Effect of different keratinocyte growth factor (KGF) concentrations on hair follicle stem cells proliferation. Cells were assessed 3 days after treatment. (A) the KGF 0 µg/L group shows cells have grown well and display a cobblestone-like appearance; (B) the KGF 10 µg/L group shows cell density has increased. A small number of enlarged cells have appeared; (C) the KGF 25 µg/L group shows differentiated cells with enlarged nuclei and enriched cytoplasm can be seen; (D) the KGF 50 µg/L group shows the proportion of differentiated cells increased, and the cell density decreased; (E) the KGF 100 µg/L group shows the proportion of differentiated cells increased, cell aging was observed. Magnification, X100