fig6

Figure 6. Effect of different LiCl concentrations on hair follicle stem cells proliferation. Cells were assessed 3 days after treatment of LiCl at different concentrations. (A) after treatment with 0 mmol/L LiCl, cells have grown well and display a cobblestone-like appearance; (B) after treatment with 0.5 mmol/L LiCl, cells have become rounded, or occasionally an elongated spindle shape, and show a tendency to aggregate; (C) after treatment with 1.5 mmol/L LiCl, in general, cells have become larger. Occasionally, cells have developed protuberances, which may be related to cell migration; (D) after treatment with 10 mmol/L LiCl, cell morphology has changed. Cells have become highly aggregated and cell density is reduced; (E) after treatment with 25 mmol/L LiCl, cell density has significantly decreased. Both the cytoplasm and nuclei show various morphological changes. The proliferation rate has also decreased. Magnification, X100