fig5
Figure 5. LAMP-1+ lysosomal accumulation in FD podocytes visualized through automated image analysis. (A) Representative images of a FD podocyte after 10 days of differentiation under brightfield, (B) CFP, and (C) GFP channels, showing plasma membrane, nucleus, and LAMP-1+ lysosomes. (D-F) Real-time automated cellular analysis using Harmony software to identify the: (D) nucleus, (E) cytoplasm, and (F) number and area of LAMP-1+ vesicles (termed spots) in FD podocytes. (G) Real-time localization of LAMP-1+ lysosomes in FD podocytes over time of differentiation on days 10 and 20. (H) Quantification of the mean number of LAMP-1+ lysosomes per cell; and (I) LAMP-1+ lysosome number as a proportion of total cell area in FD versus control podocytes at day 10 and day 20 time points. Scale bars represent 50 µm. Data are presented as mean ± SEM. Statistical significance is indicated as
