fig3

Figure 3. Dysregulation of lysosomal proteins in FD podocytes. (A) Gene ontology (GO) term enrichment analysis of FD podocytes, highlighting significantly enriched pathways related to cellular components, biological processes, and molecular functions. Several genes were involved in these pathways, indicated by the increasing size of the colored circles and significantly enriched proteins were identified based on a false discovery rate < 0.05. (B) Volcano plot showing differential protein abundance in FD podocytes relative to controls, with upregulated proteins in FD podocytes (blue) and downregulated proteins (red) highlighted. (C) A schematic representation of the lysosome showing specific proteins that are upregulated (green) and downregulated (red) in FD podocytes relative to controls. n = 4 replicates per iPSC-podocyte line. CAV1: Caveolin 1; COL6A1: collagen type IV alpha 1 chain; CTSB: cathepsin B; DAGLB: diacylglycerol lipase beta; GALC: galactosylceramidase; GBA: glucosylceramindase; GLA: galactosidase alpha; GO: Gene Ontology; GPC4: glypican 4; HSPG2: heparan sulfate proteoglycan 2; LIPA: lipase A; LUM: lumican; NPC2: Niemann-Pick disease type C2 protein; OCIAD2: OCIA domain-containing protein 2; OCLN: occludin; PLK1: polo-like kinase 1; SRC: proto-oncogene tyrosine-protein kinase; UBXN6: UBX domain-containing protein 6; VASN: vasorin.