fig5

Figure 5. Comparison of Y-SNP reads in maternal plasma across different groups: the non-pregnant control group (red points), the female fetal group (green points), and the male fetal group (blue points). Each data point corresponds to a single Y-SNP read. The average Y-SNP reads for each group are depicted as a red line. The predefined threshold of 30× is represented by a dotted line. There was a statistically significant difference (P < 0.001) in Y-SNP reads of the male fetal group and the other groups, as indicated by *** and determined by an independent-sample t-test.