fig2

Figure 2. Method 3 produced a larger quantity and higher quality of retinal organoids. (A) Brightfield images show proper differentiation of retinal domains pre-excision (arrowheads indicate retinal domains). (B) More organoids per differentiation were acquired using Method 3 compared to Method 2. (C) Whole cryosections of 85-day-old retinal organoids from each differentiation method, stained with an early photoreceptor marker (CRX) and counterstained with DAPI are shown. (D) The relative CRX-positive area was greatest in the retinal organoids differentiated using Method 3. (E) Whole cryosections of 85-day-old retinal organoids stained with a ganglion cell marker (BRN3A) and counterstained with DAPI are shown. (F) The relative BRN3A positive area was greatest in the retinal organoids differentiated using Method 3. CRX: Cone-rod homeobox; BRN3A: POU class 4 homeobox. Scale bar (A) 100 µm; (C, E) 300 µm. (B, D, F) *P < 0.017 (Bonferroni-corrected).