fig5

Figure 5. CD4⁺ T cells in canine glioma patients before and after treatment with M032. (A, B) Numbers of CD4⁺ Foxp3^-, CD4⁺ Foxp3⁺ (Treg), and CD8⁺ T cells in the peripheral blood of (A) healthy controls vs. pre-M032 treatment canine glioma patients and (B) pre- vs. day 14 post-M032 treatment canine glioma patients. (C, D) The proportions of naïve (CD44^lo CD45RA^hi), central memory (CD44^hi CD45RA^hi), and effector/effector memory (CD44^hi CD45RA^lo) in (C) CD4⁺ and (D) CD8⁺ T cells. Two-way ANOVA for grouped analyses and Dunnet’s correction for multiple comparisons was used to calculate statistical significance. ****P < 0.0001, ***P < 0.001, **P <0.01.