fig5

Figure 5. Dietary feeding of KRE enhances H3K9 dimethylation and inhibits cell proliferation in vivo. (A, B) Western blot analysis of H3K9 dimethylation in mouse dorsolateral prostates of TRAMP mice and prostate cancer PDX tumors, respectively, in vehicle control and 0.6% KRE treatment groups. (C) Ki67 immunohistochemistry staining was shown in the prostates of TRAMP mice that was treated with vehicle control vs. 0.6% KRE containing food for 18 weeks. 200× magnifications of images are presented. (D) The percentage of positive stained cells was calculated after 20 fields from ten mouse prostates were counted in each group. KRE: Kava root extract; H3K9: histone H3 lysine 9; TRAMP: transgenic adenocarcinoma of the mouse prostate; PDX: patient-derived xenograft; LSD1: lysine-specific demethylase 1.