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Figure 2. Differential gene expression for CD24 and SFRP4 between sample groups classified by patient age, sample type, or metastasis factors. CD24 and SFRP4 are 2 of the 36 genes in the iPAM classifier. A and B, Gene expression data from the 119 COH patients were used to generate the box and dot plots; there were significant tumor-vs.-matched-benign median expression differences among young patients (≤ 50 years) with Gleason score of 7 for CD24 (A) and SFRP4 (B) (black vs. blue for young patients, FDR < 0.05); for CD24 (panel A), median expression level was significantly higher in tumors from young patients than in tumors from old patients with Gleason score of 7 (black vs. green, FDR < 0.05). C and D, Gene expression data for 545 patients in the Mayo discovery data set were used to generate plots; among young (≤ 55 years) patients, significantly increased median expression levels for CD24 (C) and SFRP4 (D) were observed in patients with metastasis compared to patients without metastasis (black vs. blue, FDR < 0.05); however, no significant median expression difference related to metastasis status among patients older than 70 years (red vs. green, P > 0.2).