fig2
![Exome sequencing identifies <i>FLNC</i> and <i>ADD3</i> variants in a family with cardiomyopathy](https://image.oaes.cc/be0014c4-c84f-4ead-ae87-df4d26668716/2571.fig.2.png)
Figure 2. Panel A shows photograph of ethidium bromide-stained polyacrylamide gel of the BspDI digests to validate and genotype the ADD3 mutation c.169C>T Arg57X. M is the molecular weight marker lane, U contains uncut PCR product. The arrow indicates the smaller fragment validating the mutation in the proband 141 and his daughter 1661 and not in three other family members. Panel B shows the DNA sequencing chromatogram demonstrating the ADD3 heterozygous mutation in the proband's daughter's DNA [red (T)/blue (C) double peaks in top two lines; control sequence on the bottom line]