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From: Aging triggers mitochondrial, endoplasmic reticulum, and metabolic stress responses in the heart

Figure 5. Increased oxidative stress in cardiac aging. Left: Immunoblot showing the expression of 4-HNE in aged (25-month) vs. young (10-month) CBA/CaJ mice hearts and corresponding Ponceau stained loading control blot. Right: Densitometry quantification of the 4-HNE levels normalized to ponceau staining. Relative expression values were represented as mean ± SEM (n = 5). **P < 0.01 is considered significant, calculated by an unpaired Student’s t-test using GraphPad Prism software.