fig4

Figure 4. Angiotensin II-induced vasoconstriction is attenuated in young female compared with male mice, and this difference is abolished with AT2R inhibition. Second- and third-order mesenteric resistance arteries were isolated from 3-month-old C57/Bl6 female and male mice. Vessels were pre-treated with vehicle (circles) or 10 μM PD123319 (squares), an AT2R antagonist. Vessel constriction in response to AngII (1 × 10^-7 M) was determined by wire myography. Data are expressed as raw force in milliNewtons (mN). N = 8-10/group. The main effects of treatment and sex and the interaction effect of these two factors were assessed via two-way ANOVA with Tukey post hoc testing where appropriate. Data are means ± SEM, *P < 0.05.