fig1
Figure 1. Angiotensin II-induced vasoconstriction increases later in life in female compared with male mice. Second- and third-order mesenteric resistance arteries were isolated from 3-, 12-, and 18-month-old C57/Bl6 mice. Four separate segments from each mouse were hung in a wire myograph and administered a different concentration of angiotensin II (1 × 10-9 to 1 × 10-7 M). Microvascular constriction was measured in vessels from (A) females and (B) males in response to angiotensin II concentrations by wire myography. Data are expressed as raw force in milliNewtons (mN) from baseline. N = 3-4/group (all female groups and 3-month-old males = 3/group; 12- and 18-month-old males = 4/group). The main effects of concentration and age and the interaction effect of these two factors were assessed via repeated measures two-way ANOVA with Tukey post hoc testing where appropriate. Data are means ± SEM,
