fig5

Figure 5. Enhanced Exercise by RGS14 KO Mice Is Mediated by BAT and Angiogenesis /Arteriogenesis Increasing HindLimb Blood Flow. Non-linear contrast imaging was used to measure hindlimb blood flow. The average data are presented as % of WT perfusion, which is represented as 100% (A). Hindlimb blood flow was higher in RGS14 KO mice compared to WT mice, and higher in WT mice that received RGS14 KO BAT, at 3 days after transplantation (A and B), while RGS14 KO BAT donors lost their enhanced hindlimb perfusion, with results similar to WT mice (A). With treatment of the SIRT3 inhibitor, 3-YTP, both RGS14 KO mice and RGS14 KO BAT recipients lost their enhanced hindlimb perfusion (A). Angiogenesis (reflected by capillary density) and arteriogenesis (reflected by arteriole density) were both increased in skeletal muscle of RGS14 KO mice (B and C) and RGS14 KO BAT recipients (D and E), which correlated with increased VEGF in skeletal muscle (F) and BAT (G). Reprinted from Ref.^[72].