fig7

Figure 7. Nobiletin fails to rescue autophagy gene expression and cell death in the presence of shATG7. (A) Western blot analysis of LC3 protein expression in cardiac myocytes under normoxia (NMX) and hypoxia (HPX) in the presence and absence of Nob and shATG7. Histogram represents quantitative data for the western blot, relative to α sarcomeric Actin as a loading control. (B) Cell viability assessed with vital dyes calcein-AM (green; live cells) and ethidium-homodimer-1 (red; dead cells), bar: 100 μm. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01, n = 3-4 independent myocyte isolations, counting > 200 cells for each condition tested.