fig4
Figure 4. Nobiletin upregulates autophagic function of cardiac cells during hypoxia. (A) western blot analysis of LC3 and Beclin protein expression in cardiac myocytes under normoxia (NMX) and hypoxia (HPX) in the presence and absence of Nob. Histogram represents quantitative data for the western blot, relative to α sarcomeric Actin as a loading control. (B) Upper panel, epifluorescence microscopy of cardiac myocytes expressing autophagy reporter GFP-LC3 (green puncta) with or without Chloroquine (CQ) to assess autophagic flux under normoxia (NMX) and hypoxia (HPX) in the presence and absence of Nob, bar: 10 μm, magnified regions are depicted by the yellow boxes. Bottom panel, analysis of conditions tested on upper panel. Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01,
