fig5

Figure 5. Northern blot analysis of candidate mt-tRNAs identified from RNA-seq analysis. Three mt-tRNAs were selected for measurement by northern blot in cardiac RV and LV tissue: (1)MT-TL1 (control, no change in RNA seq); (2) MT-TY (significantly decreased in RNA-seq); and (3) MT-TP (significantly increased in RNA-seq). In the RV, MT-TY showed significantly reduced abundance in the POLG mutant relative to WT controls (confirming RNA-seq finding) while MT-TP showed no change in abundance between the groups [Northern blots shown in (A) and quantified in (B)]. This pattern of mt-tRNA abundance between WT and POLG mutants was also present in the LV tissue (C and D).