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![Mitochondrial DAMPs-dependent inflammasome activation during aging induces vascular smooth muscle cell dysfunction and aortic stiffness in low aerobic capacity rats](https://image.oaes.cc/7ab68264-a037-43eb-bfce-375c3b9014e5/5208.fig.4.jpg)
Figure 4. VSMCs of the aortic media and cultured aortic VSMCs of old age LCR rats show impaired mitophagy. Representative confocal images of aortic cross sections (A, top panel) were co-stained for LAMP1 (green), TOM20 (red) and SM-α-actin (blue). The white fluorescence indicates colocalization of all proteins and active mitophagy in medial VSMCs. VSMCs (B, top panel) were co-stained for LAMP1 (green) and TOM20 (red). Bright yellow fluorescence indicates colocalization of LAMP1 and TOM20 and active mitophagy. Data are presented as mean integrated density/ROI ± SEM where n = 4 independent cell lines (A and B, bottom panels). Representative confocal images of mitophagy flux, showing bright yellow colocalization of Mtphagy-(red) and Lyso-(green) dyes (C, top panel; scale