fig1

Figure 1. Tamoxifen-inducible Cre-mediated deletion of the Dsp gene in the post-natal cardiac myocytes in mice and molecular remodeling of the intercalated discs (IDs). (A) RT-PCR analysis of the Dsp transcript levels in cardiac myocytes isolated the wild type (WT), Myh6-Mcm^Tam, and Myh6-Mcm^Tam:Dsp^F/F mice. (B) Genome browser illustration of the RNA sequencing reads mapped to each exon of the Dsp gene. (C) Immunoblots showing expression of selected ID proteins in WT, Myh6-Mcm^Tam, and Myh6-Mcm^Tam:Dsp^F/F mice. VCL is used as a control for the loading conditions. (D) Quantitative data of selected ID proteins representing the blots shown in Panel C. Data are presented as fold change (FC). Both isoforms of DSP are included in the quantitative data. The data on the test proteins are normalized to the corresponding TUBA1A or VCL levels and are compared to the corresponding WT group throughout the manuscript. (E) Immunofluorescence panels showing expression and localization of selected ID proteins. Higher magnification panels to illustrate the localization of the DSP at the desmosomes in the genotype groups are also presented. (F) Quantitative data showing surface areas occupied by the selected ID proteins shown in Panel E.