fig2

Figure 2. Flow cytometry analysis of the EV products. (A) Representative scatter plots showing the analyzed EV population. (B) Positive expression of FITC+/CD63+ EVs (dark gray) compared to isotype control (light gray). (C) Positive expression of APC+/CD81+ EVs (black) compared to isotype control (light gray). (D) The calculated CD63 and CD81 positive expression of the EV products collected at the 48 and 120 h collection time points. EVs: Extracellular vesicles; PLT: human platelet lysate; FBS: fetal bovine serum.