fig7

Figure 7. GSNOR loss of function promotes electrophysiologic maturation of miPSC-derived CMs. (A) Representative Ca²⁺ transients in iPSC^WT (top, black) and iPSC^GSNOR-/- (bottom, red) mice, under electric-field stimulation at 1 Hz and under caffeine-induced (τCaff, τCaff 0Na) at Day 10, and 21 during iPSC-CM differentiation. (B) Superimposed normalized Ca²⁺ transient amplitude from iPSC^WT and iPSC^GSNOR-/- mice at Day 10, and 21 after iPSC-CM differentiation. (C) Average Ca²⁺ peak in response to the increasing frequency of stimulation in iPSC^WT (black) and iPSC^GSNOR-/- (red) mice at Day 10 and 21 after iPSC-CM differentiation. (D) Average time to Ca²⁺ peak in response to the increasing frequency of stimulation in iPSC^WT and iPSC^GSNOR-/- mice at Day 10 and 21 after iPSC-CM differentiation. (E) Average time to half Ca²⁺ decay (tt50%) (from exponential fit) of iPSC^WT (black) and iPSC^GSNOR-/- (red) at Day 10 and 21 after iPSC-CM differentiation. (F) Relative contribution of the three Ca²⁺ transport systems (SERCA, NCX, non-NCX) in iPSC^WT and (G) iPSC^GSNOR-/- mice at Day 10 and 21 after iPSC-CM differentiation. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using two-way ANOVA. (*P < 0.0001 on Day 10; ^#P < 0.0001 on Day 21; ^†P < 0.0016 on Day 21).