fig6

Figure 6. GSNOR loss of function impacts cardiomyocyte structural maturation. (A) qRT-PCR analysis of Cardiac genes, troponin I1 and I3 (Tnni1, Tnni3), myosin heavy chain isoforms-6 (Myh6) and -7 (Myh7) in murine iPSC^WT (white circles) and iPSC^GSNOR-/- (black circles) during cardiomyocyte differentiation. (B) Representative immunofluorescence staining of Troponin I in outgrowth of beating EBs on Day 10 of murine iPSC^WT (top) iPSC^GSNOR-/- (bottom). (C) qRT-PCR analysis of Myocardial genes associated with calcium (Ca²⁺) handling and cardiac contraction/relaxation, including sarco/endoplasmic reticulum Ca²⁺ ATPase 2 [sarco(endo)plasmic reticulum Ca²⁺-ATPase, murine gene (Serca)2], troponin T2 (Tnnt2), Tnni1, Tnni3, type 2 ryanodine receptor (RyR2), Myh6, Myh7, and the Myh6/Myh7 ratio in murine iPSC^WT and iPSC^GSNOR-/-. (D) Representative immunofluorescence staining of α-actinin in outgrowths of beating iPSC^WT and iPSC^GSNOR-/- EBs on Day 10. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test. *P < 0.05.