fig5

Figure 5. GSNOR loss of function reduces Brachyury (T) and accelerates cardiomyocyte differentiation. (A) qRT-PCR analysis of t in murine iPSC^WT (white circles) and iPSC^GSNOR-/- (black circles) during cardiomyocyte differentiation. WB (B) and qRT-PCR (C) of t in murine iPSC^WT on Day 4, following 24 h of incubation with GSNO. (D) qRT-PCR analysis of T in hiPSC, on Day 2, following 24 h incubation with GSNO. (E) Percentage of beating colonies from murine iPSC^WT and iPSC^GSNOR-/- during cardiomyocyte differentiation. (F) Representative images of organoids from murine iPSC^WT (top) iPSC^GSNOR-/- (bottom) on Day 10 of cardiomyocyte differentiation. (G) qRT-PCR analysis of Cardiac mesoderm (Mesp1, Isl1) and Cardiac Progenitors genes (Nkx 2.5, Gata4) in murine iPSC^WT and iPSC^GSNOR-/- during cardiomyocyte differentiation. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test. *P < 0.05.