fig4

Figure 4. GSNOR loss of function regulates EMT-Related transcription factors in murine iPSC^GSNOR-/- and hiPSCs treated with GSNO. qRT-PCR analysis of Snail (A), Slug (B), Twist (C) and E-cadherin (D) in murine iPSC^WT (white circles) and iPSC^GSNOR-/- (black circles) during cardiomyocyte differentiation. qRT-PCR analysis of SNAIL (E) and SLUG (F) in hiPSC, on Day 2, following 24 h incubation with GSNO (brown circles). Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test. *P < 0.05.