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Figure 2. GSNOR loss of function stimulates cell proliferation and cell migration in murine iPSCs. (A) Cell viability as measured by MTT assay on Day 4 in murine iPSC^GSNOR-/- and iPSC^WT. EBs (4000-5000 cells/EB) from murine iPSC^WT (white circles) and iPSC^GSNOR-/- (black circles) were incubated with BrdU and proliferation determined following short- (3 h, B), and long-term (24 h, C) plating of different numbers of EBs (4000-5000 cells/EB). (D) Average number of murine iPSC^GSNOR-/- and iPSC^WT that migrated over a 24-h period. E, Micrograph of cells that migrated and then stained with crystal violet. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test at each time point. *P < 0.05.