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![S-nitrosoglutathione reductase (GSNOR) deficiency accelerates cardiomyocyte differentiation of induced pluripotent stem cells](https://image.oaes.cc/9466c200-450c-4e79-8107-a86263b4e662/4331.fig.1.jpg)
Figure 1. GSNOR loss of function accelerates cardiomyocyte differentiation of murine iPSCs. (A) GSNOR activity in whole heart lysates of GSNOR-/- and C57BL/6 mice. (B) Schematic representation of cardiomyocyte differentiation procedure from Day 0 to Day 6. (C) Western blot analysis of Gsnor and Hsp90 (internal control) in murine iPSCWT and iPSCGSNOR-/- during cardiomyocyte differentiation. (D) qRT-PCR analysis of Gsnor in murine iPSCWT and iPSCGSNOR-/- during cardiomyocyte differentiation. (E) qRT-PCR analysis of pluripotent genes (Oct4, Sox2, Klf4) in murine iPSCWT (open circles) and iPSCGSNOR-/- (black boxes) during cardiomyocyte differentiation. (F) Morphology of embryoid bodies from iPSCWT (top) and iPSCGSNOR-/- (bottom) over a time course of 5 days. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test. *P < 0.05.