fig1

Figure 1. GSNOR loss of function accelerates cardiomyocyte differentiation of murine iPSCs. (A) GSNOR activity in whole heart lysates of GSNOR^-/- and C57BL/6 mice. (B) Schematic representation of cardiomyocyte differentiation procedure from Day 0 to Day 6. (C) Western blot analysis of Gsnor and Hsp90 (internal control) in murine iPSC^WT and iPSC^GSNOR-/- during cardiomyocyte differentiation. (D) qRT-PCR analysis of Gsnor in murine iPSC^WT and iPSC^GSNOR-/- during cardiomyocyte differentiation. (E) qRT-PCR analysis of pluripotent genes (Oct4, Sox2, Klf4) in murine iPSC^WT (open circles) and iPSC^GSNOR-/- (black boxes) during cardiomyocyte differentiation. (F) Morphology of embryoid bodies from iPSC^WT (top) and iPSC^GSNOR-/- (bottom) over a time course of 5 days. Data are the mean ± SEM of 3 independent experiments. P-values were calculated using Student’s t-test. *P < 0.05.