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Figure 5. Effects of suppression of the cWNT pathway on adipogenesis. (A) Immunofluorescence panels depicting myocardial adipocytes as detected by the expression of perilipin-1 (PLIN1), a marker for mature adipocytes, in the experimental groups. (B) The mean number of adipocytes in each myocardial section was determined and compared among the experimental groups, showing an increased number of adipocytes upon inhibition of the cWNT pathway in the Myh6-Cre:Dsp^W/F mice (N = 5-12). (C) Transcript levels of Cebpa (CCAAT enhancer-binding protein α), Agpat1 (1-acylglycerol-3-phosphate O-acyltransferase 1), Fabp4 (fatty acid-binding protein 4), and Dgat2 (diacylglycerol O-Acyltransferase 2) genes involved in adipogenesis, as quantified by RT-PCR in the experimental groups (N = 5-7). P_G: P value for the effect of the genotype; P_Tr: P value for the effect of treatment; P_i: P value for treatment-by-genotype interaction.