fig5

Figure 5. EV-miRNAs from BMSCs after expansion in the analyzed media. (A) Principal component analysis of the ln(x) transformed pg/10⁶ BMSCs values for detected EV-miRNAs. The X- and Y-axes represent principal components 1 and 2 that explain 56.8% and 43.2% of the total variance, respectively. “r” represents the Spearman correlation coefficient; (B) Heatmap of hierarchical clustering analysis of the ln(x) transformed pg/10⁶ BMSCs values of detected EV-miRNAs, with sample clustering tree shown at the top. The color scale reflects the absolute expression levels: red shades: high expression levels; blue shades: low expression levels. F, P, and S/X represent the same media conditions as in (A). BMSCs: Bone marrow-derived mesenchymal stromal cells; F: fetal bovine serum; P: platelet lysate; S/X: serum/xeno free; EVs: extracellular vesicles.