fig7

Figure 7. MiRNAs in IFNγ-sEVs regulate gene expression of IRM and ribosome pathways, both directly and indirectly. (A) Pie charts displaying the miRNA abundance in native sEVs and IFNγ-sEVs, as determined by microRNA sequencing. The matching proportions are presented alongside the top 30 most abundant miRNAs (n = 3 samples per group), color-coded for clarity; (B) The common mirnas of the top 30 most abundant mirnas in each of the two sEVs, many of which target DAM marker genes; (C) A volcano plot showing statistically significant differentially expressed miRNAs in IFNγ-sEVs compared with native sEVs (adjusted P-values ≤ 0.05); (D) KEGG analysis of highly expressed miRNAs in native sEVs and IFNγ-sEVs; (E) KEGG analysis of significantly differentially expressed miRNAs in IFNγ-sEVs compared with native sEVs; (F) The significantly differentially expressed miRNA target maker genes of IRM; (G) The significantly differentially expressed miRNA target ribosomal genes. IFNγ: Interferon-gamma; sEVs: small extracellular vesicles; IRM: interferon-responsive microglia; DAM: disease-associated microglia; KEGG: Kyoto Encyclopedia of Genes and Genomes.