fig5

Figure 5. IFNγ-pretreated small extracellular vesicles promote inhibition of microglia activation by regulating microglial inflammatory response and ribosome activity in vitro. (A) Representative confocal images depict the morphology of IBA1-stained BV2 cells (green) cultured as negative control (A1), stimulated with LPS + IFNγ for 24 h (A2), and internalized of sEVs (red) labeled with PKH26 24 after LPS + IFNγ-stimulation (A5, A6). The partial enlargement (A3, A4, A7, A8). Scale bar, 20 μm; (B) The quantization of elongation, that is, the length of the long axis divided by the length of the short axis (n = 3 samples per group); (C and D) KEGG analysis of DEGs in the IFNγ-sEVs group compared with the native sEVs group; (E) The DEGs of the ribosome pathway in the IFNγ-sEVs group were determined by GSEA; (F) GO analysis of DEGs in the IFNγ-sEVs group compared with the native sEVs group; (G) Reactome analysis of DEGs in the IFNγ-sEVs group compared with the native sEVs group. Using one-way ANOVA (B). IFNγ: Interferon-gamma; LPS: lipopolysaccharide; KEGG: Kyoto Encyclopedia of Genes and Genomes; DEGs: differentially expressed genes; sEVs: small extracellular vesicles; GO: Gene Ontology; NS: no significant difference. ^*P < 0.05; ^***P < 0.0001.