fig3

Figure 3. Cell vitality of MDA-MB-231 cells grown on different substrates. Representative epifluorescence images and the relative histogram (n = 1,071 cells CTRL, n = 932 cells PDMS 10:1, n = 1,067 cells PDMS 50:1) showing the live/dead cells of MDA-MB-231 cells plated on dish with or without PDMS. The live cells are identified based on intracellular esterase activity (green), and the dead cells by the lack of esterase activity and non-intact plasma membrane that allows for red dye staining (red). Significance of data differences was established via Ordinary one-way Anova test (^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001, respectively). PDMS: Polydimethylsiloxane.