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Figure 2. Cell morphology and stiffness of MDA-MB-231 cells plated on different substrates. Representative optical images (A), epifluorescence images with their relative histograms (n = 1,123 cells CTRL, n = 1,663 cells PDMS 10:1, n = 1,853 cells PDMS 50:1) (B), and boxplots showing AFM force spectroscopy results (n = 60 cells CTRL, n = 63 cells PDMS 10:1, n = 62 cells PDMS 50:1) (C) of MDA-MB-231 cells grown on PDMS/without PDMS substrates coated with type I collagen. The lower and upper boundaries of the box represent Q1 (25 percentile) and Q3 (75 percentile) of the data, respectively; the horizontal bar inside the box represents the median of the data. Significance of data differences was established via the Anova Kruskal-Wallis test (^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001, respectively). PDMS: Polydimethylsiloxane; AFM: atomic force microscopy.