fig8

Figure 8. Transfer of miR-100 and miR-125b decreases CGN immunofluorescence in recipient cells. (A) Transwell co-culture experiments were performed with ΔmiR-100/miR-125b recipient cells and the indicated donor cells. After 5 days, recipient cells were immunostained as in Figure 5C. Scale bar indicates 10 mm; (B) Schematic of Transwell co-culture experiments to test growth changes in spheroids due to transfer of material from donor 2-D cells to recipient spheroids grown in 3D; (C) The increase in spheroid colony area was determined by measuring the surface area of spheroids from day 0 to day 5 after exposure to donor cells. Significance was determined using Student’s t test; n = 6. Data represent mean ± SEM with ^** indicating P < 0.01. CGN: Cingulin; SEM: scanning electron microscopy.