fig5

Figure 5. miR-100 and miR-125b contribute to enhanced 3D growth and invasiveness. (A) The indicated cell types were plated in low adherent, spheroid cultures for 3 days before transfer to growth in type I collagen and live imaging over 5 days. Scale bar indicates 500 μm; (B) The increase in colony area was determined by measuring the surface area of spheroids from day 0 to day 5. Scale bar indicates 500 μm. Representative image is a CC-CR spheroid. Surface area tracing was performed using ImageJ, shown in yellow. Significance was analyzed using one-way ANOVA tests (n = 3); (C) Increases in area due to colony projections into the collagen (invasiveness) were calculated by measuring the total surface area including projections (yellow) and subtracting the surface area of the main spheroid (red). Scale bar indicates 500 μm. Representative image is a CC-CR spheroid. Significance was analyzed using one-way ANOVA tests, data (n = 3). Data represent mean ± SEM with ^** indicating P < 0.01 and ^*** indicating P < 0.001; ns indicating not significant. ANOVA: Analysis of variance; SEM: scanning electron microscopy.