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Figure 4. Decreased Expression of CGN in Cells Expressing High Levels of miR-100 and miR-125b. (A and B) Western blots and quantitation of CGN levels in the indicated cell lines; (C) The indicated cells were fixed and immunostained with DAPI to stain nuclei (blue), Alexa Fluor 488-phalloidin to stain F-actin (green), or antibodies against CGN (magenta). Merged images are shown at the far right. Scale bar indicates 10 mm; (D) Quantification of CGN/cell for the indicated cell lines. For (B), data (mean ± SEM, n = 3) were analyzed using Student’s t test. For (C), data (mean ± SEM, n = 3) were analyzed using one-way ANOVA. For significance, ^* indicates P < 0.05, ^** indicates P < 0.01, ^*** indicates P < 0.001. CGN: Cingulin; DAPI: 4’,6-diamidino-2-phenylindole; SEM: scanning electron microscopy; ANOVA: analysis of variance.