fig3
![Transfer of <i>miR-100</i> and <i>miR-125b</i> increases 3D growth and invasiveness in recipient cancer cells](https://image.oaes.cc/ca076455-e210-47f6-8999-50ccce8446b9/evcna5043.fig.3.jpg)
Figure 3. Silencing of reporter constructs containing target 3’-UTRs for miR-100 and miR-125b. (A) Reporter constructs were generated in which the firefly luciferase open reading frame was fused to either plasmid 3’-UTR sequences (empty vector), 3’-UTR sequences containing three perfect binding sites for miR-100 or miR-125b (100/125 vector), or 3’-UTR sequences from candidate mRNA targets of miR-100 or miR-125b (experimental vector); (B) Quantification of relative luciferase levels after transfection of the indicated constructs into CC-CR cells or ΔmiR-100/miR-125b cells. Relative firefly luciferase levels were calculated by normalizing to a co-transfected internal control vector expressing Renilla luciferase. Data (mean ± SEM, n = 3) were analyzed using two-way ANOVA tests, with * indicating P < 0.05, ** indicating P < 0.01, *** indicating P < 0.001, and **** indicating P < 0.0001. SEM: Scanning electron microscopy; ANOVA: analysis of variance.